Advances in Clinical and Experimental Medicine

Title abbreviation: Adv Clin Exp Med
JCR Impact Factor (IF) – 1.727
Index Copernicus  – 152.95 pts
MNiSW – 40 pts

ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

Download original text (EN)

Advances in Clinical and Experimental Medicine

2015, vol. 24, nr 6, November-December, p. 957–964

doi: 10.17219/acem/27909

Publication type: original article

Language: English

Download citation:

  • BIBTEX (JabRef, Mendeley)
  • RIS (Papers, Reference Manager, RefWorks, Zotero)

The Analgesic, Anti-Inflammatory and Anti-Pyretic Activities of Tinospora cordifolia

Liaqat Hussain1,A,B,C,D, Muhammad S. H. Akash1,A,D,E,F, Noor-Ul Ain1,B,C, Kanwal Rehman2,D,E,F, Muhammad Ibrahim3,E

1 Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Pakistan

2 Institute of Pharmacy, Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan

3 Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad, Pakistan

Abstract

Background. Tinospora cordifolia (T. cordifolia) is a valuable resource due to its traditional uses in the treatment of pain, fever and inflammation, but no sufficient scientific literature is available online to confirm its traditional uses in these ailments.
Objectives. This study was carried out to validate the traditional uses of T. cordifolia in treating pain, inflammation and pyrexia, using albino mice as an experimental animal model.
Material and Methods. The analgesic effects of T. cordifolia extract were assessed by using the acetic acid-induced writhing test, hot plate test and tail-flick test. The carrageenan test was performed to assess anti-inflammatory potential, and anti-pyretic activity was evaluated by the brewer’s yeast-induced pyrexia method.
Results. The results showed that the T. cordifolia extract exhibited significant analgesic effects in a dose-dependent manner in the three pain models tested. The extract also exhibited significant anti-inflammatory effects in the carrageenan-induced inflammation test and antipyretic effects in the brewer’s yeast-induced pyrexia test in dose-dependent manner compared to the effects observed in the control group animals.
Conclusion. From the findings of the present study, it can be concluded that T. cordifolia extract has strong analgesic, anti-inflammatory and anti-pyretic effects. Further studies are required to investigate the therapeutic activities of the phytochemical constituents of T. cordifolia against pain, inflammation and pyrexia.

Key words

anti-inflammatory, anti-pyretic, experimental model, Tinospora cordifolia

References (56)

  1. Panchabhai TS, Kulkarni UP, Rege NN: Validation of therapeutic claims of Tinospora cordifolia, a review. Phytother Res 2008, 22, 425–441.
  2. Patel MB, Mishra S: Hypoglycemic activity of alkaloidal fraction of Tinospora cordifolia. Phytomedicine 2011, 18, 1045–1052.
  3. Grover JK, Vats V, Rathi SS, Dawar R: Traditional Indian anti-diabetic plants attenuate progression of renal damage in streptozotocin induced diabetic mice. J Ethnopharmacol 2001, 76, 233–238.
  4. Rathi SS, Grover JK, Vikrant V, Biswas NR: Prevention of experimental diabetic cataract by Indian ayurvedic plant extracts. Phytother Res 2002, 16, 774–777.
  5. Prince PSM, Menon VP: Hypoglycaemic and hypolipidemic action of alcohol extract of Tinospora cordifolia root in chemical induced diabetes in rats. Phytother Res 2003, 17, 410–413.
  6. Kar A, Choudhary BK, Bandyopadhyay NG: Comparative evaluation of hypoglycaemic activity of some Indian medicinal plants in alloxan diabetic rats. J Ethnopharmacol 2003, 84, 105–108.
  7. Prince PSM, Padmanabhan M, Menon VP: Restoration of antioxidant defence by ethanolic Tinospora cordifolia root extract in alloxan-induced diabetic liver and kidney. Phytother Res 2004, 18, 785–787.
  8. Shalam M, Harish MS, Farhana SA: Prevention of dexamethasoneand fructose-induced insulin resistance in rats by SH-10D, a herbal preparation. Indian J Pharmacol 2006, 38, 419–422.
  9. Purandare H, Supe A: Immunomodulatory role of Tinospora cordifolia as an adjuvant in the surgical treatment of diabetic foot ulcers. A prospective randomized controlled study. Indian J Med Sci 2007, 61, 347–355.
  10. Bishayi B, Roychowdhury S, Ghosh S, Sengupta M: Hepatoprotective and immunomodulatory properties of Tinospora cordifolia in CCl4 intoxicated mature albino rats. J Toxicol Sci 2002, 27, 139–146.
  11. Bhattacharya S, Pandit S, Mukherjee R, Das N, Sur TK: Hepatoprotective effect of HIMOLIV, a polyherbal formulation in rats. Indian J Physiol Pharmacol 2003, 47, 435–440.
  12. Tasduq SA, Singh K, Sethi S, Sharma SC, Bedi KL, Singh J, Jaggi BS, Johri RK: Hepatocurative and antioxidant profile of HP-1, a polyherbal phytomedicine. Hum Exp Toxicol 2003, 22, 639–645.
  13. Bhar MK, Das SK, Chakraborty AK, Mandal TK, Roy S: Hepatoprotective effect of Enliv on paracetamol-induced liver damage in broiler chicks. Indian J Pharmacol 2005, 37, 257–258.
  14. Sohni YR, Bhatt RM: Activity of a crude extract formulation in experimental hepatic amoebiasis and in immunomodulation studies. J Ethnopharmacol 1996, 54, 119–124.
  15. Samy RP: Antimicrobial activity of some medicinal plants from India. Fitoterapia 2005, 76, 697–699.
  16. Bafna PA, Balaraman R: Anti-ulcer and anti-oxidant activity of Pepticare, a herbomineral formulation. Phytomedicine 2005, 12, 264–270.
  17. Manjrekar PN, Jolly CI, Narayanan S: Comparative studies of the immunomodulatory activity of Tinospora cordifolia and Tinospora sinensis. Fitoterapia 2000, 71, 254–257.
  18. Jena GB, Kumar VS, Kaul CL, Ramarao P: Protective effect of a polyherbal formulation (Immu–21) against cyclophosphamide-induced mutagenicity in mice. Phytother Res 2003, 17, 306–310.
  19. Diwanay S, Chitre D, Patwardhan B: Immunoprotection by botanical drugs in cancer chemotherapy. J Ethnopharmacol 2004, 90, 49–55.
  20. Tadesse G, Reneela P, Dekebo A: Isolation and characterization of natural products from Helinus mystachnus (Rhamnaceae). J Chem Pharm Res 2012, 4, 1756–1762.
  21. Oduola T, Bello I, Adeosun G, Ademosun AW, Raheem G, Avwioro G: Hepatotoxicity and nephrotoxicity evaluation in Wistar albino rats exposed to Morinda lucida leaf extract. North Am J Med Sci 2010, 2, 230–233.
  22. Silva GN, Martins FR, Matheus ME, Leitão SG, Fernandes PD: Investigation of anti-inflammatory and antinociceptive activities of Lantana trifolia. J Ethnopharmacol 2005, 100, 254–259.
  23. Nwafor PA, Okwuasaba FK: Anti-nociceptive and anti-inflammatory effects of aqueous methanolic extract of Asparagus pubescens root in rodents. J Ethnopharmacol 2003, 84, 125–129.
  24. D’amour FE, Smith DL: A method for determining loss of pain sensation. J Pharmacol Exp Ther 1941, 72, 74–79.
  25. Suleyman H, Demirezer LÖ, Kuruüzüm A, Banoğlu ZN, Göçer F, Özbakir G, Gepdiremen A: Anti-inflammatory effect of the aqueous extract from Rumex patientia roots. J Ethnopharmacol 1999, 65, 141–148.
  26. Chatterjee GK, Burman TK, Nagchaudhuri AK, Pal SP: Anti-inflammatory and antipyretic activities of Morus indica. Planta Med 1983, 48, 116–119.
  27. Das S, Kanodia L: Comaprative study of anti-inflammatory activities of whole plant and fruit of Fragaria vesca in experimental animal models. J Nat Pharmaceuticals 2011, 2, 20–23.
  28. Donath MY, Boni-Schnetzler M, Ellingsgaard H, Ehses JA: Islet inflammation impairs the pancreatic b-cell in type 2 diabetes. Physiology 2009, 24, 325–331.
  29. Donath MY, Shoelson SE: Type 2 diabetes as an inflammatory disease. Nat Rev Immunol 2011, 11, 98–107.
  30. Dinarello CA: Blocking interleukin-1β acute and chronic auto-inflammatory diseases. J Intern Med 2011, 269, 16–28.
  31. Akash MSH, Rehman K, Chen S: An overview of valuable scientific models for diabetes mellitus. Curr Diabetes Rev 2013, 9, 286–293.
  32. Akash MSH, Shen Q, Rehman K, Chen S: Interleukin-1 receptor antagonist, a new therapy for type 2 diabetes mellitus. J Pharm Sci 2012, 101, 1647–1658.
  33. Akash MSH, Rehman K, Chen S: Role of inflammatory mechanisms in pathogenesis of type 2 diabetes mellitus. J Cell Biochem 2013, 114, 525–531.
  34. Bresnihan B: Treatment of rheumatoid arthritis with interleukin-1 receptor antagonist. Ann Rheum Dis 1999, 58, 196–198.
  35. Dayer JM, Feige U, Edwards CK, Burger D: Anti-interleukin-1 therapy in rheumatic diseases. Curr Opin Rheumatol 2001, 13, 170–176.
  36. Allan SM, Tyrrell PJ, Rothwell NJ: Interleukin-1 and neuronal injury. Nat Rev Immunol 2005, 5, 629–640.
  37. Lucas SM, Rothwell NJ, Gibson RM: The role of inflammation in CNS injury and disease. Br J Pharmacol 2006, 147, 232–240.
  38. Ehses JA, Lacraz G, Giroix M, Schmidlin F, Coulaud J, Kassis N, Irminger JC, Kergoat M, Portha B, Homo-Delarche F, Donath MY: IL-1 antagonism reduces hyperglycemia and tissue inflammation in the type 2 diabetic GK rat. Proc Natl Acad Sci USA 2009, 106, 13998–14003.
  39. Akash MSH, Rehman K, Li N, Gao JQ, Sun H, Chen S: Sustained delivery of IL-1Ra from Pluronic F127-based thermosensitive gel prolongs its therapeutic potentials. Pharm Res 2012, 29, 3475–3485.
  40. Akash MSH, Rehman K, Sun H, Chen S: Interleukin-1 receptor antagonist improves normoglycemia and insulin sensitivity in diabetic GK-rats. Eur J Pharmacol 2013, 701, 87–95.
  41. Akash MSH, Rehman K, Chen S: Sustained delivery of IL-1Ra from PF127 gel reduces hyperglycemia in diabetic GK rats. PLoS One 2013, 8, e55925.
  42. Akash MSH, Rehman K, Chen S: IL-1Ra and its Delivery Strategies, Inserting the Association in Perspective. Pharm Res 2013, 30, 2951–2966.
  43. Adeneye AA: Protective activity of the stem bark aqueous extract of Musanga cecropioides in carbon tetrachlorideand acetaminophen-induced acute hepatotoxicity in rats. Afr J Tradit Complement Altern Med 2009, 6, 131–138.
  44. Ajiboye TO, Salau AK, Yakubu MT, Oladiji AT, Akanji MA, Okogun JI: Acetaminophen perturbed redox homeostasis in Wistar rat liver, protective role of aqueous Pterocarpus osun leaf extract. Drug Chem Toxicol 2010, 33, 77–87.
  45. Ahmed H, Zahab HAE, Alswiai G: Purification of antioxidant protein isolated from Peganum harmala and its protective effect against CCl4 toxicity in rats. Turk J Biol 2013, 37, 39–48.
  46. Purnima A, Koti BC, Thippeswamy AHM, Jaji MS, Swamy AHMV, Kurhe YV, Sadiq AJ: Anti-inflammatory, analgesic and antipyretic activities of Mimusops elengi linn. Indian J Pharm Sci 2010, 72, 480–485.
  47. Jude EO, Anwanga EU, Samuel GF, Louis A: Anti-inflammatory and analgesic activities of Melanthera scandens. Asian Pacific J Tropical Biomed 2012, 2, 144–148.
  48. Parveen A, Akash MSH, Rehman K, Mahmood Q, Qadir MI: Analgesic, anti-inflammatory and anti-pyretic activities of Caesalpinia decapetala. Bioimpacts 2014, 4, 43–48.
  49. Hussain L, Ikram J, Rehman K, Tariq M, Ibrahim M, Akash M: Hepatoprotective effects of Malva sylvestris L. against paracetamol-induced hepatotoxicity. Turk J Biol 2014, 38, 396–402.
  50. Akash MSH, Rehman K, Rasool F, Sethi A, Abrar MA, Irshad A, Abid A, Murtaza G: Alternate therapy of type 2 diabetes mellitus (T2DM) with Nigella (Ranunculaceae). J Med Plants Res 2011, 5, 6885–6889.
  51. Rehman K, Akash MSH, Azhar S, Khan SA, Abid R, Waseem A, Murtaza G, Sherazi TA: A biochemical and histopathologic study showing protection and treatment of gentamicin-induced nephrotoxicity in rabbits using vitamin C. Afr J Tradit Complement Altern Med 2012, 9, 360–365.
  52. Akash MSH, Rehman K, Chen S: Effects of coffee on type 2 diabetes mellitus. Nutrition 2014, 30, 755–763.
  53. Akash MSH, Rehman K, Chen S: Spice plant Allium Cepa, Dietary supplement for treatment of type 2 diabetes mellitus. Nutrition 2014. DOI: 10.1016/j.nut.2014.02.011.
  54. Mossa JS, Rafatullah S, Galal AM, Al-Yahya MA: Pharmacological studies of Rhus retinorrhaea. Pharm Biol 1995, 33, 242–246.
  55. Singh SS, Pandey SC, Srivastava S, Gupta VS, Patro B, Ghosh AC: Chemistry and medicinal properties of Tinospora cordifolia (Guduchi). Indian J Pharmacol 2003, 35, 83–91.
  56. Singh RK, Bhattacharya SK, Acharya SB: Studies on extracts of Elaeocarpus sphaericus fruits on in vitro rat mast cells. Phytomedicine 2000, 7, 205–207.