Advances in Clinical and Experimental Medicine

Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2019, vol. 28, nr 4, April, p. 431–438

doi: 10.17219/acem/92563

Publication type: original article

Language: English

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Creative Commons BY-NC-ND 3.0 Open Access

Impact of fabrics from transgenic flax on cultures of skin cells

Kazimierz Gąsiorowski1,A,C,E,F, Tomasz Gębarowski1,B,C,D, Helena Moreira1,B,C, Anna Kulma2,E,F, Michał Szatkowski2,E, Jan Szopa2,A,E

1 Department of Basic Medical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Poland

2 Department of Genetic Biochemistry, Faculty of Biotechnology, University of Wroclaw, Poland

Abstract

Background. The development of a new type of wound dressing material that can support skin regeneration is an important challenge to improve treatment of chronic, non-healing wounds.
Objectives. The objective of this study was to compare the impact of flax fabrics from transgenic plants overexpressing phenolic acids and flavonoids (W92) and polyhydroxybutyrate (M48), as well as fabric from non-transgenic plant (Nike) on cultures of human skin cells.
Material and Methods. Flax fabric pieces as well as water extracts from the fabrics were co-cultured with human skin cells: keratinocytes, fibroblasts, dermal microvascular endothelial cells, and with monocytoid cell line (THP1) for 48 h. Cell viability and proliferation were assessed with the sulforhodamine B colorimetric assay. Intracellular reactive oxygen species (ROS) was estimated with the 2’7 dichlorodihydrofluorescein diacetate (DCFH-DA) oxidation method. Endothelial cell migration was measured with the scratch assay. The results were compared with the multi-criteria analysis (MCA) procedure.
Results. Tested flax fabrics released flavonoids and polyhydroxybutyrate to cell culture media, as it was determined by means of the high performance liquid chromatography (HPLC) method. Fabrics from transgenic plants W92 and M48 promoted proliferation of keratinocytes and fibroblasts. Water extracts from flax fabric diminished the proliferation of monocytoid cells, decreased oxidative burst in activated THP1 cells and accelerated the velocity of dermal microvascular cell migration. The MCA proved that the sum of beneficial effects estimated in human skin cell cultures was higher (by 47% and by 34% with W92 and M48, respectively) than that of non-transgenic flax fabric (Nike).
Conclusion. The W92 and M48 fabrics should be further studied as candidates for elaboration of new types of bandages, able to improve skin wound healing.

Key words

flax fabric, skin cell cultures, flax biotechnology, transgenic flax fabrics

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