Advances in Clinical and Experimental Medicine

Adv Clin Exp Med
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ISSN 1899–5276 (print),   ISSN 2451-2680 (online)
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Advances in Clinical and Experimental Medicine

2019, vol. 28, nr 2, February, p. 223–228

doi: 10.17219/acem/87045

Publication type: original article

Language: English

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Creative Commons BY-NC-ND 3.0 Open Access

Calophyllum inophyllum in vaginitis treatment: Stimulated by electroporation with an in vitro approach

Jerzy Zalewski1,C,E,F, Justyna Mączyńska2,B,C, Katarzyna Bieżuńska-Kusiak2,B,F, Julita Kulbacka2,A,D,F, Anna Choromańska2,C,E, Monika Przestrzelska1,E, Maciej Zalewski1,C,E, Zbigniew Saczko3,C, Łucja Cwynar-Zając4,B,E, Agnieszka Rusak4,B,E, Jolanta Saczko2,A,D,F

1 Department of Gynecology and Obstetrics, Faculty of Health Sciences, Wroclaw Medical University, Poland

2 Department of Molecular and Cellular Biology, Faculty of Pharmacy with Division of Laboratory Diagnostics, Wroclaw Medical University, Poland

3 Antoni Falkiewicz Specialized Hospital, Wrocław, Poland

4 Department of Histology and Embryology, Faculty of Medicine, Wroclaw Medical University, Poland

Abstract

Background. Vaginitis is one of the most common problems in clinical medicine and is cited most often during visits to obstetricians and gynecologists. Most of the inflammation cases are caused by candidiasis trichomoniasis and bacterial vaginosis. Therefore, treatment of vaginal infections must use antibiotic or antifungal drugs, which often provide quick relief to the patient. The real cause of the problem – disrupting the ecosystem of the vagina – remains unchanged. Thus, new therapeutic compounds are being explored.
Objectives. The aim of our study was to evaluate the effect of a natural substance: tamanu oil, an extract from the plant Calophyllum inophyllum, applied to the human fibroblast cell line (normal human dermal fibroblasts – NHDFs) and to the isolated human fibroblasts from the vagina (human vaginal fibroblasts – HVFs) in vitro.
Material and Methods. We evaluated the viability of cells by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay after incubation only with tamanu oil and with electroporation (EP). We also examined the immunocytochemical reaction of collagen type III and mitochondrial superoxide dismutase (MnSOD) under established conditions.
Results. Tamanu oil increased the proliferation of cells and the amount of collagen III. It has been shown that the C. inophyllum extract stimulates the proliferation of commercial fibroblasts. For direct application in patients, one should use C. inophyllum extract in the range of 1:10–1:100 (saline dilution).
Conclusion. The use of this extract (at concentrations indicated by the studies presented here) stimulates the healing processes (increased expression of collagen type III), and has anti-inflammatory, analgesic and antiseptic qualities.

Key words

electroporation, vaginitis inflammation, primary fibroblasts culture, tamanu oil

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