Advances in Clinical and Experimental Medicine

Adv Clin Exp Med
Impact Factor (IF) – 1.514
Index Copernicus (ICV 2018) – 157.72
MNiSW – 40
Average rejection rate – 84.38%
ISSN 1899–5276 (print)
ISSN 2451-2680 (online)
Periodicity – monthly

Download PDF

Advances in Clinical and Experimental Medicine

2019, vol. 28, nr 2, February, p. 165–169

doi: 10.17219/acem/78021

Publication type: original article

Language: English

Download citation:

  • BIBTEX (JabRef, Mendeley)
  • RIS (Papers, Reference Manager, RefWorks, Zotero)

Creative Commons BY-NC-ND 3.0 Open Access

Expression of mucins MUC5AC and MUC19 on the ocular surface in dry eye syndrome model of ovariectomized female rabbits

Guoxing Li1,A,B, Peirong Lu2,B,C, Huiyang Song1,B,C, Qinxiang Zheng1,D,E, Kaihui Nan1,D,E,F

1 Eye Hospital, Wenzhou Medical University, China

2 Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, China

Abstract

Background. Dry eye syndrome (DES) is a common symptom of tear film instability and ocular surface damage due to an abnormal quality and quantity of tears, including the sensation of foreign objects and blurred vision. Among all factors for tear film stability, MUC5AC and MUC19 are very important; the levels of both mucins are associated with the pathogenesis of DES.
Objectives. The aim of this study was to explore the expression of MUC5AC and MUC19 on the ocular surface in a DES model of ovariectomized female rabbits.
Material and Methods. Healthy female New Zealand white rabbits (n = 18; age: 1 year, weight: 2.5 ±0.6 kg) were randomly assigned to a test group and a control group. The DES model was constructed in ovariectomized female rabbits. Indicators of ocular surface injury, such as Schirmer’s test, corneal fluorescence staining, a conjunctival imprinting cytology test, and the expression of MUC5AC and MUC19 in conjunctival tissues were evaluated by immunohistochemistry in week 1, week 2 and week 4.
Results. Both the length of soaked test paper and the total scores of corneal fluorescence staining at all time-points were significantly lower in the test group than in the control group, and they decreased over time (p < 0.05). The grades of imprinted cells at all time-points were significantly higher in the test group than in the control group, and they increased over time (p < 0.05). The percentage of goblet cells was significantly lower in the test group than in the control group, and it decreased over time (p < 0.05). The percentages of cells with a positive expression of MUC5AC and MUC19 at all time-points were significantly lower in the test group than in the control group, and they decreased over time (p < 0.05).
Conclusion. The pathogenesis of DES is associated with an increased grade of imprinted cells, decreased goblet cells, and a decreased expression of MUC5AC and MUC19.

Key words

dry eye syndrome model, ovariectomized female rabbits, MUC5AC, MUC19

References (20)

  1. Wan KH, Chen LJ, Young AL. Depression and anxiety in dry eye disease: A systematic review and meta-analysis. Eye (Lond). 2016;30(12):1558–1567.
  2. Sriprasert I, Warren DW, Mircheff AK, et al. Dry eye in postmenopausal women: A hormonal disorder. Menopause. 2016;23(3):343–351.
  3. Ching-Li T, Ya-Jung H, Chen ZY, et al. Synergistic effect of artificial tears containing epigallocatechin gallate and hyaluronic acid for the treatment of rabbits with dry eye syndrome. PLoS One. 2016;11(6): e0157982.
  4. Yu GY, Zhu ZH, Mao C, et al. Microvascular autologous submandibular gland transfer in severe cases of keratoconjunctivitis sicca. Maxillofac Plast Reconstruct Surg. 2015;33:235–239.
  5. Aragona P, Pietro RD, Spinella R, et al. Conjunctival epithelium improvement after systemic pilocarpine in patients with Sjogren’s syndrome. Brit J Ophthalmol. 2006;90:166–170.
  6. Kojima T, Ishida R, Dogru M, et al. The effect of autologous serum eyedrops in the treatment of severe dry eye disease: A prospective randomized case-control study. Am J Ophthalmol. 2005;139:242–246.
  7. Yavuz B, Pehlivan SB, Kaffashi A, et al. In vivo tissue distribution and efficacy studies for cyclosporin A loaded nano-decorated subconjunctival implants. Drug Deliv. 2016;23(9):3279–3284.
  8. Feng Y, Feng G, Peng S, et al. The effect of hormone replacement therapy on dry eye syndrome evaluated with Schirmer test and break-up time. J Ophthalmol. 2015;2015:420302.
  9. Feng Y, Feng G, Peng S, et al. The effects of hormone replacement therapy on dry eye syndromes evaluated by Schirmer test depend on patient age. Cont Lens Anterior Eye. 2015;39:124–127.
  10. Erdem U, Ozdegirmenci O, Sobaci E, et al. Dry eye in post-menopausal women using hormone replacement therapy. Maturitas. 2007;56: 257–262.
  11. Ablamowicz AF, Nichols JJ. Ocular surface membrane-associated mucins. Ocul Surf. 2016;14:331–341.
  12. Uchino Y, Uchino M, Yokoi N, et al. Impact of cigarette smoking on tear function and correlation between conjunctival goblet cells and tear MUC5AC concentration in office workers. Sci Rep. 2016;6. Article number: 27699.
  13. Coursey TG, Henriksson JT, Barbosa FL, et al. Interferon-γ-induced unfolded protein response in conjunctival goblet cells as a cause of mucin deficiency in Sjögren syndrome. Am J Pathol. 2016;186:1547–1558.
  14. Lin H, Qu Y, Geng Z, et al. Air exposure induced characteristics of dry eye in conjunctival tissue culture. PLoS One. 2014;9:e87368.
  15. Zhao H, Jumblatt JE, Wood TO, et al. Quantification of MUC5AC protein in human tears. Cornea. 2001;20:873–877.
  16. Corrales RM, Narayanan S, Fernández I, et al. Ocular mucin gene expression levels as biomarkers for the diagnosis of dry eye syndrome. Invest Ophthalmol Vis Sci. 2011;52:8363–8369.
  17. Zhang J, Yan X, Li H. Analysis of the correlations of mucins, inflammatory markers, and clinical tests in dry eye. Cornea. 2013;32:928–932.
  18. Yu DF, Chen Y, Han JM, et al. MUC19 expression in human ocular surface and lacrimal gland and its alteration in Sjögren syndrome patients. Exp Eye Res. 2008;86:403–411.
  19. Watanabe H. Significance of mucin on the ocular surface. Cornea. 2002;21:17–22.
  20. Yang L, Yang Z, Yu H, et al. Acupuncture therapy is more effective than artificial tears for dry eye syndrome: Evidence based on a meta-analysis. Evid Based Complement Alternat Med. 2015;2015:143858.