Advances in Clinical and Experimental Medicine

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Advances in Clinical and Experimental Medicine

2018, vol. 27, nr 9, September, p. 1181–1193

doi: 10.17219/acem/90872

Publication type: original article

Language: English

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Creative Commons BY-NC-ND 3.0 Open Access

Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation

Katarzyna Jezierska-Woźniak1,A,B,C,D, Seweryn Lipiński2,C,D, Margaret Huflejt3,A,C,D, Łukasz Grabarczyk3,B, Monika Barczewska3,A,C, Aleksandra Habich4,B, Joanna Wojtkiewicz,C, Wojciech Maksymowicz3,A,F

1 Department of Neurology and Neurosurgery, Faculty of Medical Sciences, Laboratory for Regenerative Medicine, University of Warmia and Mazury in Olsztyn, Poland

2 Department of Electric and Power Engineering, Electronics and Automatics, Faculty of Technical Sciences, University of Warmia and Mazury in Olsztyn, Poland

3 Center of Innovative Research in Medical and Natural Sciences, Faculty of Medicine, University of Rzeszów, Poland



Background. The analysis of the stem cells’ glycome dynamics at different stages of differentiation and migration makes possible the exploration of the cell surface glycans as markers of the stem cell functional status, and, in the future, compatibility between transplanted cell and host environment.
Objectives. The objective of our study was to develop novel techniques of investigating cell motility and to assess whether the electric field of the therapeutic spinal cord stimulation system used in vivo contributes to the migration of human mesenchymal stem cells (hMSCs) in vitro.
Material and Methods. We have investigated the electrotaxis of bone marrow-derived MSCs using pulsed electric field (PEF) in the range of 16–80 mV/mm and the frequency of 130 Hz and 240 Hz. The PEF-related dynamics of the cell surface glycosylation was evaluated using 6 plant lectins recognizing individual glycans.
Results. Pulsed electric field at physiological levels (10 mV/mm; 130 Hz) did not influence cellular motility in vitro, which may correspond to the maintenance of the transplanted cells at the lesion site in vivo. An increase of the PEF intensity and the frequency exceeding physiological levels resulted in an increase in the cellular migration rate in vitro. Pulsed electric field elevated above physiological intensity and frequency (40–80 mV/mm; 240 Hz), but not at physiological levels, resulted in changes of the cell surface glycosylation.
Conclusion. We found the described approach convenient for investigations and for the in vitro modeling of the cellular systems intended for the regenerative cell transplantations in vivo. Probing cell surface glycomes may provide valuable biomarkers to assess the competence of transplanted cells.

Key words

translational medicine, mesenchymal stem cells migration, stem cells homing, pulsed electric field, stem cell glycosylation

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