Advances in Clinical and Experimental Medicine

Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2018, vol. 27, nr 11, November, p. 1567–1572

doi: 10.17219/acem/74653

Publication type: original article

Language: English

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Creative Commons BY-NC-ND 3.0 Open Access

Circulating parameters of oxidative stress and hypoxia in normal pregnancy and HELLP syndrome

Ahmet Mentese1,A,B,C,D,F, Süleyman Güven2,A,D,F, Selim Demir3,B,C,D, Ayşegül Sümer4,B,C, Serap Özer Yaman5,B,C, Ahmet Alver5,A,E,F, Mehmet Sonmez6,A,E,F, Süleyman Caner Karahan5,A,E,F

1 Program of Medical Laboratory Techniques, Vocational School of Health Sciences, Karadeniz Technical University, Trabzon, Turkey

2 Department of Obstetrics and Gynecology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey

3 Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, Trabzon, Turkey

4 Department of Nursing, School of Health Services, Recep Tayyip Erdogan University, Rize, Turkey

5 Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey

6 Department of Hematology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey

Abstract

Background. The HELLP syndrome (Hemolysis, Elevated Liver enzymes and Low Platelets) is a complication of severe pre-eclampsia, a condition characterized by oxidative stress elevation caused by disequilibrium between lipid peroxidation and antioxidant defense mechanisms, which, in turn, results in endothelial compromise and free radical-mediated cell damage. While several studies have examined the relationship between pre-eclampsia and oxidative stress, research investigating oxidative and hypoxic status in HELLP syndrome is limited.
Objectives. The aim of this study was to compare the levels of oxidative stress markers – total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), and malondialdehyde (MDA) – and a hypoxia marker – carbonic anhydrase IX (CA IX) – in patients with HELLP syndrome and in healthy pregnant women.
Material and Methods. A total of 23 women with HELLP syndrome and 30 healthy pregnant women were included in the study. Serum levels of oxidative stress markers were determined using colorimetric methods, while serum levels of CA IX were measured using an enzyme-linked immunosorbent assay (ELISA) kit.
Results. The TOS, OSI, MDA, and CA IX levels were significantly higher in women with HELLP syndrome than in the controls (p = 0.0001, p = 0.0001, p = 0.0001, and p = 0.008, respectively).
Conclusion. Increased levels of oxidative stress and hypoxia markers in women with HELLP syndrome suggest that oxidative stress and hypoxia may be significantly involved in the pathophysiology of the disease. Further follow-up studies are now needed to investigate the prognostic roles of these parameters in patients with HELLP syndrome.

Key words

oxidative stress, carbonic anhydrases, hypoxia, HELLP syndrome

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