Advances in Clinical and Experimental Medicine

Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2017, vol. 26, nr 2, March-April, p. 263–268

doi: 10.17219/acem/67716

Publication type: original article

Language: English

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Creative Commons BY-NC-ND 3.0 Open Access

Frequency of infection with Helicobacter pylori isolates of different antimicrobial profiles in children and adolescents: A preliminary study

Grażyna Gościniak1,A,D, Monika M. Biernat1,C,D,E, Aldona Bińkowska1,B, Agnieszka Kus1,B,C, Barbara Iwańczak2,B

1 Department of Microbiology, Wroclaw Medical University, Poland

2 2nd Department of Pediatrics, Gastroenterology and Nutrition, Wroclaw Medical University, Poland

Abstract

Background. Helicobacter pylori (H. pylori) infection can occur as a mixed infection caused by several strains of H. pylori.
Objectives. The aim of the study was to determine the frequency of colonization of the gastric mucosa by strains of H. pylori with different susceptibility to antimicrobial agents.
Material and Methods. The study was carried out on gastric biopsies taken from 54 previously untreated Polish children and adolescents. Of the 15 positive cultures, from each primary medium, 6 single H. pylori colonies were isolated, making a total of 90 isolates, and the susceptibility to metronidazole (MZ), amoxicillin (AC) and clarithromycin (CH) was determined by E-test method. The presence of the cagA gene and vacA alleles (s1, s2, m1, m2) was determined by PCR.
Results. Positive culture for H. pylori was noted in 15/54 (27.7%) of patients. All H. pylori isolates were susceptible to AC, 27.8% were resistant to MZ and 38.9% to CH. The results showed 7/15 (46.7%) of children were infected with H. pylori strains with antibiotic heteroresistance, resistant to CH (5/15, 33.3%) and to MZ (2/15, 13.3%). The cagA + vacA s1/m2 combination was predominant genotype among detected H. pylori strains. The isolates possessing different antimicrobial susceptibility profiles in the same patient were identified.
Conclusion. Microbiological analyses confirmed the presence of isolates possessing different antimicrobial susceptibility profiles in 47% of examined children with H. pylori infection. Different antimicrobial susceptibility profiles of H. pylori isolates detected in the same patient may influence the success of eradication therapy.

Key words

mixed infections, resistance, genotypes

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