Advances in Clinical and Experimental Medicine
2016, vol. 25, nr 4, July-August, p. 673–679
Publication type: original article
Alpha-Tocopherol May Protect Hepatocytes Against Oxidative Damage Induced by Endurance Training in Growing Organisms
1 Department of Human Nutrition, Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences, Poland
2 Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Poland
Background. Training-induced oxidative stress can be reduced by α-tocopherol. Adequate intake of α-tocopherol could have health benefits for previously untrained young subjects.
Objectives. The aim of this study was to determine the effects of training and different doses of α-tocopherol on exercise-induced oxidative stress in rat livers.
Material and Methods. Young male Wistar rats (n = 40) were randomly divided into eight groups (undergoing training and not undergoing training, given orally administered α-tocopherol doses of 0, 0.5, 1.0 and 4.0 mg). Every day for 10 consecutive days, the rats in the training groups ran for 15 min on a treadmill at 20 m/min to induce oxidative stress. Hepatic oxidative stress was evaluated based on the liver concentrations of 8-hydroxy-2’-deoxyguanosine (8-OHdG) and thiobarbituric acid reactive substances (TBARS).
Results. The liver concentrations of α-tocopherol were significantly influenced by α-tocopherol doses (p < 0.001) and physical exercise (p < 0.001). The liver concentrations of α-tocopherol increased in response to the highest dose (4 mg/d) of α-tocopherol in the non-training groups. In the training groups, the liver concentrations of α-tocopherol were independent of the dose. The levels of TBARS, a marker of lipid peroxidation, were lowest in the training and non-training rats administered 4.0 mg of α-tocopherol. Physical exercise and α-tocopherol doses significantly influenced TBARS concentrations (p = 0.004, p < 0.05).
Conclusion. The results of this study indicate that running training causes lipids peroxidation and reduces α-tocopherol levels in the liver, but it does not contribute to DNA damage. Increased liver concentrations of α-tocopherol were found to exert a protective effect against oxidative damage induced by endurance training. An adequate intake of α-tocopherol is important for previously untrained young subjects.
oxidative stress, rats, 8-OHdG, α-tocopherol, TBARS
- Wasserman DH, Cherington AD: Hepatic fuel metabolism during muscular work, role and regulation. Am J Physiol 1991, 260, 811–824.
- Hoene M, Weigert C: The stress response of the liver to physical exercise. Exerc Immunol Rev 2010, 16, 163–183.
- Bloomer RJ, Goldfarb AH, McKenzie MJ: Oxidative stress response to aerobic exercise, comparison of antioxidant supplements. Med Sci Sports 2006, 38, 1098–1105.
- Huang HY, Helzlsouer KJ, Appel LJ: The effect of vitamin C and vitamin E on oxidative DNA damage, results from a randomized controlled trial. Cancer Epidemiol Biomarkers Prev 2000, 9, 647–652.
- Möller P, Loft S: Dietary antioxidants and beneficial effect on oxidatively damaged DNA. Free Radic Biol Med 2006, 41, 388–415.
- Zaremba T, Oliński R: Oksydacyjne uszkodzenia DNA – ich analiza oraz znaczenie kliniczne. Post Bioch 2010, 56, 124–138.
- Ogonovszky H, Sasvári M, Dosek A, Berkes I, Kaneko T, Tahara S: The effects of moderate, strenuous and overtraining on oxidative stress markers and DNA repair in rat liver. Can J Appl Physiol 2005, 30, 186–195.
- Choi EY, Cho YO: The effects of physical training on antioxidative status under exercise-induced oxidative stress. Nutr Res Pract 2007, 1, 14–18.
- Liu J, Yeo CH, Övervik-Douki HT, Doniger JS, Chu WD, Brooks AG, Ames NB: Chronically and acutely exercise rats, biomarkers of oxidative stress and endogenous antioxidants. J Appl Physiol 2000, 89, 21–28.
- Taysi S, Oztasan N, Efe H, Polat MF, Gumustekin K, Siktar E: Endurance training attenuates the oxidative stress due to acute exhaustive exercise in rat liver. Acta Physiol Hung 2008, 95, 337–334.
- Claycombe KJ, Meydani SN: Vitamin E and genome stability. Mut Res 2001, 475, 37–44.
- Avellini L, Chiaradia E, Gaiti A: Effects of exercise training, selenium and vitamin E on some free radical scavengers in horses. Comp Biochem Phys B 1999, 123, 147–154.
- Sacheck JM, Decker EA, Clarcson PM: The effect of diet on vitamin E intake and oxidative stress in response to acute exercise in female athletes. Eur J Appl Phys 2000, 83, 40–46.
- Jordão AA, Chiarello PG, Arantes MR, Meirelles MS, Vannucchi H: Effect of an acute dose of ethanol on lipid peroxidation in rats, action of vitamin. Food Chem Toxicol 2004, 42, 459–464.
- Uchida T, Abe C, Nomura S, Ichikawa T, Ikeda S: Tissue distribution of α- and γ-tocotrienol and γ-tocopherol in rats and interference with their accumulation by α-tocopherol. Lipids 2012, 47, 129–139.
- Wawrzyniak A, Górnicka M, Hamułka J, Gajewska M, Drywień M, Pierzynowska J, Gronowska-Senger A: α-tocopherol, ascorbic acid and β-carotene protect against oxidative stress but reveal no direct influence on p53 expression in rats subjected to stress. Nutr Res 2013, 33, 868–875.
- Wawrzyniak A, Hamułka J, Drywień M, Górnicka M, Pierzynowska J, Wojtaś M, Gajewska M, Frąckiewicz J, Gronowska-Senger A: Antioxidant vitamins as oxidative stress markers in rat plasma after physical exercise. Polish J Food Nutr 2014, 64, 277–281.
- Reeves PG: Components of the AIN-93 diets as improvements in the AIN-76A diet. J Nutr 1997, 127, 838–841.
- Gronowska-Senger A, Górnicka M, Kołodziejska K: Tocopherol acetate vs. oxidative stress induced by physical exercise in rats. Pol J Food Nutr Sci 2009, 59, 263–269.
- Katsanidis E, Addis PB. Novel HPLC analysis of tocopherols, tokotrienols and cholesterol in tissue. Free Rad Biol Med 1999, 27, 1137–1140.
- Ohkawa H, Ohishi N, Yagi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979, 95, 351–358.
- Siomek A, Gackowski D, Rozalski R, Dziaman T, Szpila A, Guz J: Higherleukocyte 8-oxo-7,8-dihydro-2’-deoxyguanosine and lower plasma ascorbate in aging humans? Antioxid Redox Signal 2007, 9, 143–150.
- Mastaloudis A, Morrow JD, Hopkins DW, Devaraj S, Traber MG: Antioxidant supplementation prevents exerciseinduced lipid peroxidation, but not inflammation, in ultramarathon runners. Free Rad Biol Med 2004, 36, 1329–1341.
- Bucioli S, de Abreu CL, Valenti WE, Vannucchi H: Effects of vitamin E supplementation on renal non-enzymatic antioxidants in young rats submitted to exhaustive exercise stress. BMC Complem Altern Med 2011, 11, 23–31.
- Sureda A, Tauler P, Aquilo A, Cases N, Llompart I, Tur JA, Pons A: Antioxidant supplementation influences the neutrophil tocopherol associated protein expression, but not the inflammatory response to exercise. Cen Eur J Biol 2007, 2, 56–70.
- Duthie SJ, Gardner PT, Morrice PC, Wood SG, Pirie L, Bestwick CC: DNA stability and lipid peroxidation in vitamin E-deficient rats in vivo and colon cells in vitro. Eur J Nutr 2005, 44, 195–203.
- Kinoshita S, Tsuji E: Vitamin E supplementation attenuates strenuous exercise induced DNA damage and lipid peroxidation of the liver in rats. Kawasaki J Med Welfare 2008, 14, 1–7.
- Codoner-Franch P, Muniz P, Gasco E, Domingo JV, Valls-Belles V: Effect of a diet supplemented with α-tocopherol and β-carotene an ATP and antioxidant levels after hepatic ischemia-reperfusion. J Clin Biochem Nutr 2008, 43, 13–18.
- Pozzi R, Rosa JC, Eguchi R, Oller do Nascimento CM, Oyama LM, Aguiar O Jr: Genetic damage in multiple organs of acutely exercised rats. Cell Biochem Funct 2010, 28, 632–636.
- Fisher-Wellman K, Bloomer RJ: Acute exercise and oxidative stress, a 30 year history. Dyn Med 2009, 8, 1–25.
- Radak Z, Kumagai S, Nakamoto H, Goto S: 8-Oxoguanosine and uracil repair of nuclear and mitochondrial DNA in red and white skeletal muscle of exercise-trained old rats. J Appl Physiol 2007, 102, 1696–1701.
- Nakamoto H, Kaneko T, Tahara S, Hayashi E, Naito H, Radak Z, Goto S: Regular exercise reduces 8-oxodG in the nuclear and mitochondrial DNA and modulates the DNA repair activity in the liver of old rats. Exp Geerontol 2007, 42, 287–295.
- Ohkuwa T, Itoh H, Yamamoto T, Minami C, Yamazaki Y, Kimoto S, Yoshida R: Effects of hypoxia and hypoxic training on 8-hydroxydeoxyguanosine and glutathione levels in the liver. Metabolism 2004, 53, 716–719.
- Llorente-Cantarero FJ, Gil-Campos M, Benitez-Sillero JD, Muñoz-Villanueva MC, Tasset I, Pérez-Navero JL: Profile of oxidant and antioxidant activity in prepubertal children related to age, gender, exercise, and fitness. Appl Physiol Nutr Met 2013, 38, 421–426.