Advances in Clinical and Experimental Medicine
2015, vol. 24, nr 2, March-April, p. 227–232
Publication type: original article
Antioxidant Effect of Ethanolic Extract of Propolis in Liver of L-NAME Treated Rats
1 Department of Biology, Faculty of Arts and Sciences, Nigde University, Nigde, Turkey
2 Department of Chemistry, Faculty of Arts and Sciences, Inonu University, Malatya, Turkey
3 Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Inonu University, Malatya, Turkey
4 Department of Medicinal and Aromatic Plants, Vocational of Technical Sciences, Askaray University, Turkey
5 Department of Chemistry, Faculty of Arts and Sciences, Mus Alparslan University, Mus, Turkey
Background. The blocking of nitric oxide synthase (NOS) activity may cause vasoconstriction with formation of reactive oxygen species. Propolis is a natural product collected from plants by honeybees. Propolis has biological and pharmacological properties.
Objectives. This study was designed to investigate the effects of propolis on catalase (CAT) activity, nitric oxide (NO) and malondialdehyde (MDA) levels in the liver tissues of NOS inhibited rats by Nω-Nitro-L-arginine methyl ester (L-NAME).
Material and Methods. Rats were given a NOS inhibitor (L-NAME, 40 mg/kg, intraperitoneally) for 15 days to provoke hypertension and propolis (200 mg/kg, by gavage) the last 5 of the 15 days.
Results. Nitric oxide levels in the liver tissue of the rats given L-NAME significantly decreased (p < 0.01). That parameter did not significantly alter in the liver of rats treated with propolis compared to the control group. CAT activity and MDA levels in the liver of the rats administrated L-NAME significantly increased compared to the control group (p < 0.01). These parameters significantly decreased in the liver of the rats given L-NAME + propolis compared to the L-NAME group (p < 0.01).
Conclusion. The present data shows that L-NAME in the liver may enhance oxidative stress via inhibited nitric oxide synthase. Our results also suggest that this effect is suppressed by the antioxidant properties of propolis in the liver tissue of NOS inhibited rats.
L-NAME, liver, oxidative stress, propolis, rat.
- Guix FX, Uribesalgo I, Coma M, Munoz FJ: The physiology and pathophysiology of nitric oxide in the brain. Prog Neurobiol 2005, 76, 126–152.
- Correia Miranda LE, Capellini VK, Reis GS, Celotto AC, Carlotti Jr CG, PRB: Effects of partial liver ischemia followed by global liver reperfusion on the remote tissue expression of nitric oxide synthase: lungs and kidneys evora. Transpl P 2010, 42, 1557–1562.
- Farahini H, Ajami M, Razaz JM, Azad N, Soleimani M, Ayatollahi SA, Abotaleb N, Peyrovi H, Pazoki-Toroudi H: Nitric oxide is necessary for diazoxide protection against ischemic injury in skeletal muscle. Iran J Pharm Res 2012, 11, 375–381.
- Hong H, Sun J, Cai W: Multimodality imaging of nitric oxide and nitric oxide synthases. Free Rad Biol Med 2009, 47, 684–698.
- Jaffri JM, Mohamed S, Ahmad IN, Mustapha NM, Manap YA: Nordanial Rohimi Effects of catechin-rich oil palm leaf extract on normal and hypertensive rats’ kidney and liver. Food Chem 2011, 128, 433–441.
- Mittal M, Gupta T, Lee F, Sieber C, Groszmann RJ: Nitric oxide modulates hepatic vascular tone in normal rat liver. Am J Phys 1994, 267, 416–422.
- Dey A, Cederbaum AI: Alcohol and oxidative liver injury. Hepatology 2006, 43, 63–74.
- Jovanovic Z, Jovanovic S: A comparison of the effects of cumene hydroperoxide and hydrogen peroxide on retzius nerve cells of the leech Haemopis sanguisuga. Exp Anim 2013, 62, 9–17.
- Alirezaei M, Dezfoulian O, Kheradmand A, Neamati Sh, Khonsari A, Pirzadeh A: Hepatoprotective effects of purified oleuropein from olive leaf extract against ethanol-induced damages in the rat. Iran J Vet Res 2012, 13, 218–226.
- Shen W, Shı D, Wand D, Guo Y, Hai S, Yue Z: Quinestrol treatment ınduced testicular damage via oxidative stress in male mongolian gerbils (Meriones unguiculatus). Exp Anim 2011, 60, 445–453.
- Haq IUI, Ullah N, Bibi G, Kanwal S, Ahmad MS, Mirza B: Antioxidant and cytotoxic activities and phytochemical analysis of euphorbia wallichii root extract and its fractions. Iran J Pharm Res 2012, 11, 241–249.
- Wang HK: The therapeutic potential of flavonoids. Expert Opin Invest Drug 2000, 9, 2103–2119.
- Erdogan S, Ates B, Durmaz G, Yilmaz I, Seckin T: Pressurized liquid extraction of phenolic compounds from Anatolia propolis and their radical scavenging capacities. Food Chem Toxicol 2011, 49, 1592–1597.
- Talas ZS, Gulhan MF: Effects of various propolis concentrations on biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss). Ecotox Environ Safe 2009, 72, 1994–1998.
- Gogebakan A, Talas ZS, Ozdemir I, Sahna E: Role of propolis on tyrosine hydroxylase activity and blood pressure in nitric oxide synthase-inhibited hypertensive rats. Clin Exp Hypertens 2012, 34, 424–428.
- Lowry O, Rosebrough NJ, Farr AL, Randall RJ: Protein measurements with the folin phenol reagent. J Biol Chem 1951, 193, 265–275.
- Aebi H: Catalase in vitro assay methods. Methods Enzymol 1984, 105, 121–126.
- Cortas NK, Wakid NW: Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin Chem 1990, 36, 1440–1443.
- Yagi K: Assay of lipid peroxidation in blood plasma or serum. Methods Enzymol 1984, 105, 328–331.
- Harrison DG, Gongora MC, Guzik TJ, Widder J: Oxidative stress and hypertension. J Am Soc Hypertens 2007, 1, 30–44.
- Deniz E, Colakoglu N, Sari A, Sonmez MF, Tugrul I, Oktar S, Ilhan S, Sahna E: Melatonin attenuates renal ischemia–reperfusion injury in nitric oxide synthase inhibited rats. Acta Histochem 2006, 108, 303–309.
- Deniz E, Sahna E, Aksulu HE: Nitric oxide synthase inhibition in rats: Melatonin reduces blood pressure and ischemia/reperfusion-induced infarct size. Scand Cardiovasc J 2006, 40, 248–252.
- Sahna E, Deniz E, Bay-Karabulut A, Burma O: Melatonin protects myocardium from ischemia-reperfusion injury in hypertensive rats: Role of myeloperoxidase activity. Clin Exp Hypertens 2008, 30, 673–681.
- Maruyama H, Sumitou Y, Sakamoto T, Araki Y, Hara H: Antihypertensive effects of flavonoids isolated from brazilian gren propolis in spontaneously hypertensive rats. Biol Pharm Bull 2009, 32, 1244–1250.
- Satoshi M: Antihypertensive effects of Brazilian propolis: Identification of caffeoylquinic acids as constituents involved in the hypotension in spontaneously hypertensive rats. Biol Pharm Bull 2005, 28, 1909–1914.
- Bernátová I, Pechánová O, Babál P, Kyselá S, Stvrtina S, Andriantsitohaina R: Wine polyphenols improve cardiovascular remodeling and vascular function in NO-deficient hypertension. Am J Physiol Heart Circ Physiol 2002, 282, 942–948.
- Prytzyk E, Dantas AP, Salomao K, Pereira AS, Bankova VS, Castro SL, Aquino Neto FR: Flavonoids and trypanocidal activity of Bulgarian propolis. J Ethnopharmacol 2003, 88, 189–193.
- Schmidt RJ, Baylis C: Total nitric oxide production is low in patients with chronic renal disease. Kidney Int 2000, 58, 1261–1266.
- Geckil H, Ates B, Durmaz G, Erdogan S, Yilmaz I: Antioxidant, free radical scavenging and metal chelating characeristics of propolis. Am J Biochem Biotech 2005, 1, 27–31.
- Harborne JB, Williams CA: Advances in flavonoid research since 1992. Phytochemistry 2000, 55, 481–504.
- Selamoglu Talas Z: Propolis reduces oxidative stress in L-NAME-induced hypertension rats. Cell Biochem Funct 2014, 32, 150–154.
- Sousa T, Pinho D, Morato M, Marques-Lopes J, Fernandes E, Afonso J, Oliveira S, Carvalho F, Albino-Teixeira A: Role of superoxide and hydrogen peroxide in hypertension induced by an antagonist of adenosine receptors. Eur J Pharmacol 2008, 588, 267–276.
- Gulhan MF, Duran A, Talas ZS, Kakoolaki S, Mansouri SM: Effects of propolis on microbiologic and biochemical parameters of rainbow trout (Oncorhynchus mykiss) after exposure to the pesticide. Iran J Fish Sci 2012, 11, 490–503.
- Talas ZS, Dundar SP, Gulhan MF, Orun I, Kakoolaki S: Effects of propolis on some blood parameters and enzymes in carp exposed to arsenic. Iran J Fish Sci 2012, 11, 405–414.
- Tanasiewicz M, Skucha-Nowak M, Dawiec M, Krol W, Skaba D, Twardawa H: Influence of Hygienic Preparations with a 3% Content of Ethanol Extract of Brazilian Propolis on the State of the Oral Cavity. Adv Clin Exp Med 2012, 21, 1, 81–92.