Advances in Clinical and Experimental Medicine

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Advances in Clinical and Experimental Medicine

2014, vol. 23, nr 2, March-April, p. 159–167

Publication type: original article

Language: English

Expression of the PAR-1 Protein on the Surface of Platelets in Patients with Chronic Peripheral Arterial Insufficiency – Preliminary Report

Urszula Jakobsche-Policht1,A,B,C,D, Dorota Bednarska-Chabowska1,B,E, Anna Sadakierska-Chudy2,B, Joanna Adamiec-Mroczek3,F

1 Department and Clinic of angiology, Hypertension and diabetology, Wroclaw Medical University, Poland

2 Molecular Techniques Unit, Wroclaw Medical University, Poland

3 Department and Clinic of Ophthalmology, Wroclaw Medical University, Poland

Abstract

Background. The activation of pro-coagulation mechanisms associated with the vascular wall’s immune and inflammatory responses wall to injury plays a crucial role in the mechanisms of the induction and progression of atherosclerosis.
Objectives. The aim of this study was to determine the role of protease activated receptors (PaR-1) expressed on the surface of blood platelets in the pathogenesis of chronic peripheral arterial obliterative disease (PaOd) in patients with obliterative atherosclerosis (n = 24) and diabetic macroangiopathy (n = 16), as well as in the controls (n = 12).
Material and Methods. In addition to the expression of PaR-1, serum/plasma concentrations of thrombin-antithrombin complex (TaT), the von Willebrand factor (vWf), the platelet-derived growth factor, monocyte chemotactic protein, the soluble form of the platelet endothelial cell adhesion molecule, thrombin activatable fibrinolysis inhibitor and interleukin 6 (IL-6) were determined.
Results. Compared to the controls, PaOd patients were characterized by significantly higher levels of PaR-1 expression, vWf, TaT and IL-6. Individuals with diabetic macroangiopathy did not differ significantly from individuals with obliterative atherosclerosis in terms of PaR-1 expression. Upon activation with thrombin receptor antagonist peptide (TRaP), the levels of PaR-1 were comparable in all analyzed groups. In patients with diabetic macroangiopathy, a significant association was observed between the expression of PaR-1 on the surface of the platelet and the serum TaT concentration, as well as between TaT and serum IL-6 concentration.
Conclusion. enhanced expression of PaR-1 on the thrombocyte surface in chronic PaOd patients occurs equally in cases of diabetic macroangiopathy and in individuals free from this endocrine pathology.

Key words

adhesion molecules, diabetic macroangiopathy, obliterative atherosclerosis, PaR-1.

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