Advances in Clinical and Experimental Medicine

Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2013, vol. 22, nr 5, September-October, p. 621–627

Publication type: original article

Language: English

Protective Effects of Melatonin and β-d-Glucan Against Liver Injury in Rats – a Comparative Study

Działanie ochronne melatoniny i β-d-glukanu w uszkodzeniach wątroby u szczurów – studium porównawcze

Mustafa Said Aydogan1,A,C,D, Mehmet Ali Erdogan1,D,E, Aladdin Polat2,B, Aytac Yücel1,E, Ulkü Ozgül1,E, Hakan Parlakpinar3,B, Zeynep Rumeysa Duran2,B, Azibe Yildiz4,C, Mahmut Durmus1,D

1 Department of Anesthesiology and Reanimation

2 Department of Physiology

3 Department of Pharmacology

4 Department of Histology, Inonu University, Faculty of Medicine, Malatya, Turkey

Abstract

Objectives. The aim of this study was to investigate the possible protective effects of melatonin and β-d-glucan against ischemia-reperfusion (IR) injury in rats.
Material and Methods. Forty rats were randomly divided into 5 groups, each consisting of 8 animals, as follows. Sham group [S], IR group [C], IR + β-Glucan group [β], IR + melatonin group [MLT], IR + melatonin + β-Glucan group [MLT + β]. The rats in the C, β, MLT and MLT + β groups were subjected to IR for 60 min each. Melatonin (10 mg∙kg–1) was intraperitoneally injected for a single dose 30 min before IR. β-Glucan (50 mg∙kg–1∙day–1) was orally administered for 10 days to rats. All of the rats were killed on day 11, and histological changes in the liver and tissue levels of oxidants and antioxidants were evaluated.
Results. Malondialdehyde [MDA] level were significantly higher in the C group compared to the S group (p = 0.007). MDA level were significantly higher in the β group compared to the MLT and MLT + β groups (p =0.007). Tissue antioxidant markers (superoxide dismut ase [SOD], glutathione-peroxidase [GPx], and catalase [CAT]) were significantly lower in the C group than the S group (p < 0.05). SOD levels were simply not significant in the β group compared to the MLT and MLT + β groups. CAT and GPx activities were significantly higher in the β group compared to the MLT and MLT + β groups (p = 0.004).The histological damage ameliorated in β, MLT and MLT + β groups compared to C group.
Conclusion. Our results suggest that melatonin and β-glucan combination pretreatment suppressed oxidative stress and increased antioxidant levels in an experimental rat model of liver IR injury.

Key words

liver, ischemia reperfusion injury, melatonin, β-glucan, oxidative stress.

Słowa kluczowe

wątroba, szkodzenie niedokrwienno-reperfuzyjne, melatonina, β-glukan, stres oksydacyjny.

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