Advances in Clinical and Experimental Medicine

Adv Clin Exp Med
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Advances in Clinical and Experimental Medicine

2013, vol. 22, nr 2, March-April, p. 157–163

Publication type: original article

Language: English

Effect of Beta-Glucan on Intestinal Anastomoses in a Rat Model

Wpływ betaglukanu na zespolenia jelitowe w modelu szczurzym

Kasim Caglayan1,A,D,F, Nilsen Erdogan2,C, Bahattin Avci3,C, Bulent Gungor4,B,E,F, Hamza Cinar4,B,D, Nilden Arslan5,C

1 Bozok University, Faculty of Medicine, Department of Surgery, Yozgat, Turkey

2 Bozok University, Faculty of Medicine, Department of Pathology, Yozgat, Turkey

3 Ondokuz Mayis University, Faculty of Medicine, Department of Biochemistry, Samsun, Turkey

4 Ondokuz Mayis University, Faculty of Medicine, Department of Surgery, Samsun, Turkey

5 Ondokuz Mayis University, Faculty of Medicine, Department of Public Health, Samsun, Turkey

Abstract

Objectives. The authors aimed to investigate the effect of â-Glucan on healing of an experimental left-sided colon anastomosis model.
Material and Methods. Twenty adult male Wistar albino rats were randomized into two groups which had colonic transection and end-to-end anastomosis. Group I (Control): anastomosis group, received no treatment (n = 10); group II, anastomosis + β-Glucan (50 mg/kg/day within seven days after surgical procedure). Bursting pressure, hydroxyproline levels and histopathological characteristics of the anastomosis were analyzed.
Results. The average burst pressure of Groups I and II were 106.67 ± 5.00 and 148.00 ± 11.35 mm Hg and hydroxyproline levels were 0.85 ± 0.14 and 1.45 ± 0.46 µg/mg, respectively. Both the burst pressure and hydroxyproline levels in group II were statistically significantly higher (p < 0.05). Histopathological examination revealed less epithelial damage in group II (p < 0.05). Though not statistically significant, less edema and damage to the submucosal-muscular layer was seen in Group II (p = 0079).
Conclusion. Due to significant increases in anastomotic bursting pressures and tissue hydroxyproline levels and considering the inhibitory effect of β-Glucan on epithelial damage, edema, and submucosal-muscular layer damage, β-Glucan was thought to contribute to the healing of the anastomosis.

Streszczenie

Cel pracy. Autorzy zbadali wpływ β-glukanu na gojenie eksperymentalnego modelu lewostronnego zespolenia okrężnicy.
Materiał i metody. Dwadzieścia dorosłych samców szczurów albinosów szczepu Wistar podzielono losowo na dwie grupy, u których wykonano przecięcie okrężnicy i zespolenie koniec do końca. Grupa I (kontrolna): wykonano zespolenie, bez leczenia (n = 10), grupa II, wykonano zespolenie i podano β-glukan (50 mg / kg / dzień w ciągu siedmiu dni po zabiegu). Oceniono ciśnienie rozrywające, stężenie hydroksyproliny i cechy histopatologiczne zespolenia.
Wyniki. Średnie ciśnienie rozrywające grupy I i II wynosiło 106,67 ± 5,00 i 148,00 ± 11,35 mm Hg, a stężenie hydroksyproliny wynosiło 0,85 ± 0,14 i 1,45 ± 0,46 ng / mg. Zarówno ciśnienie rozrywające, jak i stężenie hydroksyproliny w grupie II były statystycznie istotnie większe (p < 0,05). Badanie histopatologiczne wykazało mniejsze uszkodzenie nabłonka w grupie II (p < 0,05). Chociaż nie było to istotne statystycznie, mniejszy obrzęk i uszkodzenie warstwy podśluzówkowo-mięśniowej zaobserwowano w grupie II (p = 0.079).
Wnioski. Ze względu na znaczny wzrost ciśnienia rozrywającego i stężenia hydroksyproliny w tkankach i biorąc pod uwagę hamujący wpływ β-glukanu na uszkodzenie nabłonka, obrzęk i uszkodzenie warstwy podśluzówkowo-mięśniowej, uważa się, że β-glukan może przyczynić się do wygojenia zespolenia.

Key words

colon anastomosis, β-Glucan, burst pressure, hydroxyproline.

Słowa kluczowe

zespolenie jelita grubego, β-glukan, ciśnienie rozrywające, hydroksyprolina.

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