Advances in Clinical and Experimental Medicine

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Advances in Clinical and Experimental Medicine

2011, vol. 20, nr 6, November-December, p. 711–715

Publication type: original article

Language: English

The Effects of Anti-Adhesive Low Molecular Weight Na-Hyaluronate and Octreotide on Tissue Strength

Wpływ środków przeciwzrostowych hialuronianu sodu o małej masie cząsteczkowej i oktreotydu na wytrzymałość tkanek

Baris D. Yildiz1,, Serdar Topaloglu2,, Fatih M. Avsar1,, Fatih M. Avsar3,, Ibrahim Sakcak1,, Barlas Sulu3,, Ayse F. Avsar4,

1 Sixth Department of Surgery, Ankara Numune Training and Research Hospital, Ankara, Turkey

2 Department of General Surgery, Karadeniz Technical University Faculty of Medicine, Trabzon, Turkey

3 Department of General Surgery, Kafkas University Faculty of Medicine, Kars, Turkey

4 Department of Obstetrics and Gynecology, Atatürk Training and Research Hospital, Ankara, Turkey

Abstract

Background. The use of locally administered agents to prevent intraperitoneal adhesions is a popular research topic. The presumed effects of these agents on tissue matrix limit their use.
Objectives. To determine the effects of low-molecular-weight Na-hyaluronate and octreotide on tissue tensile strength and hydroxyproline levels. Materials and Methods. Sprague-Dawley rats were anesthetized with ketamine and 10 longitudinal incisions, 2 to 3 cm in length each, were made in the right parietal peritoneum. A 2 × 1 cm peritoneal layer was excised from the left abdominal wall during the first laparotomy. Before closing the abdomen, intraperitoneal irrigation was performed, either with Na-hyaluronate solution at a concentration of 0.25 mg/kg (n = 10) or with octreotide solution at a dosage of 5 μg/ml (n = 10). On the 14th day after the operation, the abdomen was opened and abdominal adhesions were examined. Part of the abdominal wall (4 × 4 cm in size, including midline incision) was removed to measure the tensile strength and hydroxyproline level of the tissue on the suture line.
Results. Low-molecular-weight Na-hyaluronate significantly reduced the grade of adhesion (p = 0.005). Octreotide administration inhibited adhesion formation, although not to a statistically significant degree (p > 0.05). Tissue tensile strength and hydroxyproline levels were not affected by treatment with either LMW Na-hyaluronate or octreotide (p > 0.05).
Conclusion. Na-hyaluronate and octreotide, which are well known anti-adhesive agents, do not exert any negative effects either on tissue tensile strength or hydroxyproline level.

Streszczenie

Wprowadzenie. Zapobieganie zrostom w otrzewnej za pomocą miejscowo podawanych środków jest popularnym tematem badań. Przypuszczalny wpływ tych leków na macierz tkankową może ograniczyć ich zużycie.
Cel pracy. Określenie wpływu hialuronianu sodu o małej masie cząsteczkowej i oktreotydu na wytrzymałość tkanek na rozciąganie i stężenie hydroksyproliny.
Materiał i metody. Szczury szczepu Sprague-Dawley znieczulono ketaminą i wykonano 10 nacięć wzdłużnych po 2–3 cm długości na prawej otrzewnej ściennej. Warstwę 2 × 1 cm otrzewnej wycięto z lewej ściany jamy brzusznej podczas pierwszej laparotomii. Przed zamknięciem jamy brzusznej podano dootrzewnowo roztwór hialuronianu sodu w stężeniu 0,25 mg / kg (n = 10) i roztwór oktreotydu w dawce 5 μg/ ml (n = 10). 14. dnia po operacji otwarto brzuch zwierząt i zbadano obecność zrostów w jamie brzusznej. Pobrano skrawek ściany brzucha o wielkości 4 × 4 cm, w tym nacięcie na linii środkowej, do pomiaru wytrzymałości na rozciąganie i stężenia hydroksyproliny w tkance w linii szwów.
Wyniki. Hialuronian sodu o małej masie cząsteczkowej istotnie statycznie zmniejszył intensywność powstawania zrostów (p = 0,005). Podanie oktreotydu nieistotnie statystycznie zahamowało powstawanie zrostów (p > 0,05). Podanie hialuronianu sodu o małej masie cząsteczkowej ani oktreotydu nie wpłynęło na wytrzymałość na rozciąganie tkanek i stężenie hydroksyproliny (p > 0,05).
Wnioski. Hialuronian sodu i oktreotyd, które są dobrze znanymi środkami przeciwzrostowymi nie wywierają negatywnego wpływu na wytrzymałość na rozciąganie tkanek ani stężenie hydroksyproliny.

Key words

hyaluronate, octreotide, abdominal surgery, adhesion, tissue strength

Słowa kluczowe

hialuronian, oktreotyd, chirurgia jamy brzusznej, zrost, wytrzymałość tkanki

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